Table 2. Oligomer sequence, T_m values and inhibition of luciferase activity data by LNAs and LNA–DNA chimeras that target the start site of the luciferase mRNA.

ID	Sequence 5′→3′	Tm (°C) DNA:DNA	Tm (°C) LNA:DNA	Inhibition (%)
				25 nM	100 nM	200 nM
XI	GtcTTccaTTTTAcC	50	65	13	10	0
XII	GTCTTCCATTTTACC	50	89	60	73	81
XIII	CgtcTtccaTtttaCcaacAgtacC	70	74	2	0	12
XIV	CGtCTtcCATttTacCAaCagTACC	64	ND	38	52	62
XV	GTCTtccatttTACC	50	64	29	58	78
XVI	TTTTggcgtctTCCA	48	68	12	42	49
XVII	GTCTTCcattttacC	50	60	72	78	86
XVIII	GTCTTCCattttacC	50	66	74	79	87
XIX	GTCTTccattttacC	50	56	0	0	19
XX	GTCTtccattttacC	50	53	0	0	0
XXI	GTCttccattttACC	50	60	30	52	73
XXII	GTCTtccattttACCA	50	66	43	53	68
XXIII	GCGTCttccattttACCAA	60	67	75	90	96
XXIV	GTCTTCCAtttta⁁c⁁c	50	68	38	51	62
XXV	g⁁t⁁cttccATTTTACC	50	67	0	3	48

Capitalized letters within a sequence represent LNA bases, lower case letters represent DNA bases, ⁁ indicates phosphorothioate linkages and underlined bases depict mismatches relative to luciferase mRNA. ND, T_m not determined because it was too high to accurately measure.