Table 3. Oligomer sequence, Tm values and inhibition of luciferase activity data for LNA–DNA chimeras that target the downstream coding region of luciferase mRNA.
ID | Sequence 5′→3′ | Tm (°C) DNA:DNA | Tm (°C) LNA:DNA | Inhibition (%) | ||
---|---|---|---|---|---|---|
25 nM | 100 nM | 200 nM | ||||
XXVI | GtcgTtCgCGGGCgC | 61 | 77 | 23 | 40 | 55 |
XXVII | TGtAgccATcCaTCC | 47 | 78 | 0 | 0 | 0 |
XXVIII | CGGTtccatccTCTA | 55 | 69 | 60 | 61 | 78 |
XXIX | TTGTattcagcCCAT | 52 | 68 | 33 | 39 | 69 |
XXX | GTCGttcgcggGCGC | 71 | 81 | 82 | 94 | 95 |
XXXI | GCTGttcgcggCGGC | 69 | 78 | 6 | 7 | 8 |
XXXII | TGTAgccatccATCC | 47 | 78 | 29 | 39 | 53 |
Capitalized letters within a sequence represent LNA, lower case letters represent DNA bases and underlined bases depict mismatched bases relative to luciferase mRNA.