Table 1.

Decreased maturation of DC co-cultured with T cells activated in presence of anti-CD4 Ab

	IL-12 p40 production	CD40 expression
Donor 1
DCs alone	< 4	10
DCs + T-cell stimulated	1628	49.1
DCs + T-cell stimulated + anti-CD4 Ab	1000	27.4
DCs + T-cell stimulated + control Ab	2008	44.8
Donor 2
DCs alone	13	9.7
DCs + T-cell stimulated	2453	49.6
DCs + T-cell stimulated + anti-CD4 Ab	809	2.2
DCs + T-cell stimulated + control Ab	1824	52.4

CD4⁺ T cells purified from 2 individuals were incubated with Ab against the domain 1 of the CD4 molecule or control Ab (both Ab at 10 μg/mL) or left untreated. After 1 hour, cells were washed and stimulated with anti-CD3/CD28 beads. After 24 hours, T cells were mixed with autologous monocyte-derived DCs at a ratio of 5:1, as described in the legend of Figure 1. IL-12 p40 production (expressed in pg/mL) and CD40 expression on DCs (expressed in MFI) were analyzed as described in Figure 1.