Table 2.

Effect of graded doses of exogenous rCD154 on production of immune regulatory cytokines

	IL-12 p40	IL-10
Donor 1
DCs alone	< 4	< 10
DCs + untreated T cells	4008	5260
DCs + AT-2 HIV exposed T cells	1755	2172
DCs + AT-2 HIV exposed T cells + 1 μg/mL rCD154	4725	6458
DCs + AT-2 HIV exposed T cells + 2.5 μg/mL rCD154	5118	6942
DCs + AT-2 HIV exposed T cells + 5 μg/mL rCD154	5154	7091
Donor 2
DCs alone	< 4	< 10
DCs + untreated T cells	3013	539
DCs + AT-2 HIV exposed T cells	1400	93
DCs + AT-2 HIV exposed T cells + 0.5 μg/mL rCD154	2246	633
DCs + AT-2 HIV exposed T cells + 1 μg/mL rCD154	4050	1141
DCs + AT-2 HIV exposed T cells + 2.5 μg/mL rCD154	4018	2098
DCs + AT-2 HIV exposed T cells + 5 μg/mL rCD154	4461	3644

Purified CD4⁺ T cells from 2 individuals were stimulated with anti-CD3/CD28 beads, in the presence of AT-2 HIV (AT-2—exposed T cells) or in the absence of AT-2 HIV (untreated), as described in Figure 1. After several washes, T cells were cultured with autologous DCs in the absence or presence of graded doses of rCD154 (0.5-5 μg/mL). Production of IL-12 p40 and IL-10 (expressed in pg/mL) was analyzed as described in the legend of Figure 1.