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. 2002 Dec 1;30(23):5261–5268. doi: 10.1093/nar/gkf658

Figure 7.

Figure 7

Figure 7

Release of pRb-induced effects by Skip and Ski. (A) The effects of Skip and Ski on the G1 block from pRb. pRb null Saos-2 cells were co-transfected with GFP and pRb expression plasmids (1 µg), together with Skip and Ski (5 µg). After 48 h, the cells were assessed by flow cytometry. The results are plotted as the percentage change in the G1 fraction relative to cells transfected with vector alone. The results show collated results from at least three independent transfections. Standard deviations are shown. (B) The effects of Skip and Ski on the flat cell phenotype induced by pRb: Saos-2 cells were transfected with 1 µg of pBabe-Puro expression plasmid and 5 µg each of the indicated plasmids. Transfected cells were subjected to puromycin selection for 7–10 days, and the percentage of flat cells was determined by SA-β-Gal assay. Percent flat cells indicates the total number of SA-β-Gal-positive cells divided by the total number of cells counted, and represents the average from three independent experiments. Standard deviations are shown.