Figure 3.

Schematic representation of expression constructs. The sequence within the 5′ UTR is presented and the CAT initiation codon is shown in bold. The 5′ UTR derived from plasmid pSP65(A)₁₈ is shown as a thin line whereas the 5′ UTR derived from the HD gene is shown as a thick line. The CAT ORF is denoted by a box with radial shading and the HD uORF is denoted by a box with horizontal shading. The mutation that converts the ATG codon of the uORF into a TTG codon is denoted by an asterisk [HD^–145(A-125T)/CAT], whereas the insertion of a guanine residue immediately after the ATG codon is shown by a caret [HD^–145(insG-121)/CAT]. Partial nucleotide sequence of the HD 5′ UTR is shown in lowercase letters, whereas sequence derived from the vector polylinker is shown in uppercase letters. CAT* refers to the predicted product obtained from HD(InsG^–121)/CAT, where the CAT ORF is fused in-frame with sequences derived from the HD 5′ UTR.