Table 3. Results of genetic complementation assays.

Construct		Growth at 43°Ca
1	Wild type	++
2	F18A	++
3	F22A	++
4	R46H	++
5	R57A	++
6	R62A	++
7	K66A	++
8	R67A	++
9	R70A	++
10	F18A/F22A	+
11	R62A/R67A	–
12	R62A/R70A	–
13	R67A/R70A	–
14	F18A/R62A	+
15	F18A/K66A	+
16	F18A/R67A	–
17	F22A/R62A	+
18	F22A/K66A	++
19	F22A/R67A	+
20	R46H/R57A	++
21	R46H/R62A	–
22	R46H/K66A	–
23	R46H/R67A	–
24	R46H/R70A	–

^aIf the complementation of NHY322 (i.e., growth at 43°C) observed with a mutant derivative was comparable to that of wild type C5 protein, the mutant was classified as ++; if the mutant derivative behaved like NHY322 cells transformed with pBR322, it was categorized as –. Mutant derivatives which exhibited intermediate behavior in terms of complementation were defined as +.