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. 2002 Dec 1;30(23):5103–5109. doi: 10.1093/nar/gkf671

Figure 4.

Figure 4

Assessment of the extent to which the chromatin samples have naked DNA protruding from their ends. Chromatin (H5–) or naked DNA samples were treated with exonuclease III for the times indicated under identical conditions; then the purified DNA samples were treated with S1 nuclease. Lanes M contained a 123 bp DNA ladder. (A) The mobilities of 2686, 1230 and 246 bp DNA fragments are indicated. The small apparent increase in size of the band labeled 2686 bp resulted from a slight frowning of the gel. (B) DNA from the same sample as used in (A) was further digested with AlwNI, located 805 bp from the left end. The mobilities of the 805, 1881 (the remaining portion of the 2686 bp original fragment) and 123 bp fragments are indicated. (C) DNA from the same sample as used in (A) was further digested with XmnI, located 804 bp from the right end. The mobilities of the 804, 1882 (the remaining portion of the original 2686 bp fragment) and 123 bp fragments are indicated.