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. 2002 Dec 1;30(23):5222–5228. doi: 10.1093/nar/gkf672

Figure 3.

Figure 3

Determination of the platinum binding sites of the intrastrand cross-link G*AG* and the interstrand cross-links G*AG/CUC*, obtained upon pairing G*AG* with CUC in 0.5 mM MgCl2. Denaturing gel electrophoresis of the oligonucleotides before (lanes NT) and after (lanes OH–) limited alkaline hydrolysis in 100 mM carbonate buffer pH 9.2 in the presence of 0.5 mg/ml tRNA at 100°C for 4 min. Lanes G*AG refer to the intrastrand cross-link. Lane 1 refers to cross-linked duplexes, 32P-labelled at the 5′-end of the platinated strand. Lane 2 refers to cross-linked duplexes, 32P-labelled at the 5′-end of the complementary strand.