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Amplification of PstI–MseI fragments containing SSRs corresponding to sequence tags. Each PCR was performed in replicate using six independently prepared unenriched (UE) and SSR-enriched (E) amplicon pools as template DNA. The sequence tag primers used in each reaction are: (A) STM 43, (B) STM 409 and (C) STM 639. Putative target fragments are indicated by an arrow. A 25 bp DNA ladder (Gibco BRL) was used as a size marker (M).
