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MALDI-TOF mass spectrum of cyanoethylated yeast tRNAPhe digested with RNase A in the presence of 3-HPA. The circled peak represents the 34–39 fragment that has lost the nucleobase from the wybutosine by acidic hydrolysis. The cyanoethylated version of this fragment is labelled in italics (see text for details). The table lists the expected RNase A fragments based on data present in the tRNA database (27).
