Table 1.

Primer Sequences and PCR Conditions Used for Genotyping^[Note]

	Primer(5′→3′)
Loci	Forward	Reverse	ATa(°C)	Size(bp)
SHOX (CA)n	CATGTCATATATATATGTGATCC	GACACAGAAATCCTTCATAAA	55	141–155
SHOXa/b +3/1239 and SHOXa/b +3/1248b	TTTAACCGAAATGAAGCCGAGA	AAATGTGTGTCGTGGGAGTGG	57	230
SHOX-SNP657 and SHOX-SNP792b	ATTGATGGTTAGTATTTTTTGTAGCAGTTG	TTAAAAATAAAGTTACAAAGGCCGGG	65	681
rs5988407	TGGCAAATGGATCACTTCAA	CTTGGGGTTTCCCAGAAAAA	56	376
rs7055778	AGGAGTGAGTATATCAAGACTTGGG	AGCTCTGCCTTGAGGTTGGA	58	99
rs5946324	AAAATCACCGTCGTTTGGGAG	AGATCATTTACAGCAGAAGCCG	58	195
rs5946325	AGGTGATTAGGGAAGCTCCGTGTT	TCGCTGTGCTGGAAGCAGAA	60	162
rs5946326	ACGTTAAGGGAAATGAGCCA	TTTGTCTCTTTTCAGGACTAAGGA	56	110
rs5988281, rs5946329, rs5988432, and rs4946331c	GAAACAGGACAATACCAGTGCAGT	AATCACACTGATGTGAAATTGGG	60	576
rs5988437	TGTTCTGTAAGTTGCATGTCTGCT	TGCTTTTCCTGGGTAGAAGGTA	58	224
rs6644384	AAAGAAACAAGAGCTGCGTTCC	TCTATACGGGGGCTTGAAATGT	56	280
rs5946336	CTTATTTGCCCCATCTCAGC	AAAAAGAGGAATTGTCATTCTATGT	60	103
rs7067102 and rs5946533b	TTTGCATCAGGAATCCATTTG	CCAAAGTAATGCGTAGACTTTG	60	384
rs4504827	GAAATGCAAAATAGTGGCCGAG	CTTTTACGCATTGGATCCAGTG	60	276
rs5988299, rs5988300, rs5988301, and rs5988494c	AGGATCCCAGACGCCACTCAA	GCATTCATCTGCTGCAGGAATT	58	261
DXYS233	TGGGAATTCGAGGCTG	TGATTTCCATCCTGGGGT	54	271–299
rs4468091	TCTCAAACTCAGGGTTGTTTCG	AATTCCTACAGCTATAGGTCCCAG	62	337
rs5946712	GGAATTATAGGCTGAGATCCACAG	ACAACCCTACAAATTCTAGCCCTC	58	223
DXYS85	TTTGCTGAGCACCTAGAAGG	TAGGTCCTCTAGGTGCAGGA	60	78/82

Note.— SHOX (CA)_n, DXYS233, and DXYS85 have been genotyped by determining the PCR product sizes on an ABI PRISM 310 autosequencer with GeneScan software (Applied Biosystems). Other SNPs have been genotyped by direct sequencing of the PCR products on a CEQ 8000 autosequencer (Beckman Coulter).

^aAT = annealing temperature.

^bTwo polymorphisms are present in the PCR products.

^cFour polymorphisms are present in the PCR products.