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. 2005 Nov 16;78(1):28–37. doi: 10.1086/498879

Figure 6.

Figure 6

Results showing that F187L Lfng does not modulate ligand-induced Notch signaling. A, Wild-type Lfng significantly potentiates activation of Notch1 by coculture with Dll1-expressing cells, whereas F187L and D202A mutants do not alter levels of Notch1 activation. Three asterisks (***) denote P<.001 for wild-type versus F187L Lfng. B, Wild-type Lfng significantly inhibits activation of Notch1 by coculture with Jagged1-expressing cells, whereas F187L and D202A mutants do not alter levels of Notch1 activation. Three asterisks (***) denote P<.001 for wild-type versus F187L Lfng. Notch1 cells were cotransfected with plasmids encoding wild-type Lfng, mutant Lfng, or CAT (as a negative control) and a CSL reporter. Transfected cells were cocultured with either control NIH3T3, Dll1, or Jagged1 cells and were assayed for luciferase activity. Fold activation of ligand-expressing cells over control NIH3T3 cells is expressed relative to that of CAT-transfected cocultures. Notch signaling was activated 4–10-fold in these experiments. Error bars represent SDs of four independent experiments.