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. Author manuscript; available in PMC: 2006 Oct 1.
Published in final edited form as: Biol Reprod. 2005 Jun 15;73(4):695–702. doi: 10.1095/biolreprod.105.039859

FIG.4.

FIG.4

Effect of treatment of primary amniotic epithelial cells with INSL4 for 24 h (n=4, different patients). Cytograms of flow cytometric analysis: A. control, untreated cells, B. staurosporine (STS) induced apoptosis C. INSL4 treatment, showing that INSL4 also induced apoptosis and necrosis. D. quantitation of the flow cytometry data means ± SD, showing a dose-related effect of INSL4 on induction of apoptosis. Asterisks show STS significantly (p<0.05) induced apoptosis compared to the controls, INSL4 (50ng/ml) significantly induced apoptosis (p<0.05). E. Nuclear matrix protein (NMP) measurement in the medium of the experiments shown in D as means ± SD, reflect the effect of INSL4 on causing apoptosis and necrosis. Asterisks show significantly increased (p<0.05) NMP compared to the controls.