Table I. Subcellular distribution of NADPH oxidase components p47phox and p67phox and cyclooxygenase 2 (COX2) mRNA levels in spleen.
Protein expression data are shown as the fold change relative to Ucp2+/+ without LPS treatment, which is set to 1.00 (italicized) (n = 3). Northern blotting data for RNA are shown as the fold change relative to Ucp2+/+ littermates setting to 1.00 (italicized) (n = 3).
| NADPH oxidase (protein)
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| p47phox |
p67phox |
|||||||||
| Membrane
|
||||||||||
| LPS (4 μg/g bw, 6 h)
|
Cytosol
|
Membrane
|
Cytosol
|
COX2 (mRNA)
|
||||||
| Genotypes | − | + | − | + | − | + | − | + | − | + |
| Ucp2+/+ | 1.00 ± 0.02 | 1.78 ± 0.02 | 1.00 ± 0.08 | 2.29 ± 0.14 | 1.00 ± 0.05 | 1.95 ± 0.02 | 1.00 ± 0.01 | 1.42 ± 0.02 | 1.00 ± 0.05 | 1.00 ± 0.07 |
| Ucp2−/− | 1.00 ± 0.01 | 1.49 ± 0.02 | 1.08 ± 0.08 | 1.98 ± 0.15 | 1.01 ± 0.01 | 2.09 ± 0.03 | 1.04 ± 0.01 | 1.75± 0.02 | 0.96 ± 0.04 | 1.09 ± 0.11 |