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Kinetic Parameters for TEM-1 Wild Type and the S130G Mutant
|
Km (mM)
|
kcat (s−1)
|
kcat/Km(M−1 s−1)
|
||||
|---|---|---|---|---|---|---|
| TEM-1 | S130G | TEM-1 | S130G | TEM-1 | S130G | |
| ampicillin | 0.02 | 0.13 ± 0.01 | 1530 | 170 ± 4 | 7.7 × 107 | (1.3 ± 0.1) × 106 |
| benzylpenicillin | 0.02 | 0.28 ± 0.02 | 2000 | 15 ± 1 | 1.0 × 108 | (5 ± 0.4) × 104 |
| nitrocefin | 0.22 | 0.09 ± 0.01 | 920 | 10 ± 1 | 4.2 × 106 | (1.0 ± 0.1) × 105 |
| cephaloridine | 0.93 | 0.39 ± 0.05 | 1500 | 0.7 ± 0.1 | 1.6 × 106 | (1.7 ± 0.2) × 103 |
