Table 3.
LSMean values with Standard errors of the TIMP1 gene transcripts. Depicts the LSMean values with Standard errors of the TIMP1 gene transcripts expressed by macrophages over time and measured by Real Time RT PCR as described in materials and methods.
Strain | Time post infection | ||||
2 hour | 16 hour | 24 hour | 48 hour | 96 hour | |
B1018 | 6.45 ± 0.28 | 16.65 ± 8.57 | 6.2 ± 0.09 | 11.63 ± 1.42 | 45.9 ± 8.74 |
S7565 | 12.4 ± 1.88 | 2 ± 0.67 | 5.74 ± 1.9 | 9.26 ± 0.5 | 25.2 ± 0.25 |
Hu6 | 3.12 ± 0.79 | 0.87 ± 0.16 | 1.1 ± 0.34 | 2.3 ± 0.3 | 34.6 ± 5.7 |
M. avium | 2.62 ± 0.18 | 3.2 ± 1.5 | 3.5 ± 0.07 | 4.2 ± 0.36 | 6.4 ± 1.25 |
PC1x | 6.1 ± 0.6 | 3.8 ± 0.04 | 10.7 ± 1.52 | 36.4 ± 3.39 | 159.7 ± 1.57 |
PC2y | 6.3 ± 0.34 | 24 ± 1.53 | 2.2 ± 0.65 | 52.3 ± 6.91 | 38.6 ± 5.1 |
NCz | 6.2 ± 0.91 | 26.6 ± 3.91 | 49.8 ± 23.26 | 584.2 ± 71.4 | 8.1 ± 3.46 |
x indicates positive control 1 (PC1) – cells stimulated with LPS
y indicates positive control 2 (PC2) – cells stimulated with LPS in conjunction with recombinant bovine Interferon gamma
z indicates negative control (NC) – unstimulated cells