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. Author manuscript; available in PMC: 2006 Feb 27.
Published in final edited form as: FASEB J. 2004 Jul 9;18(12):1424–1426. doi: 10.1096/fj.04-1743fje

Figure 1.

Figure 1.

Formation of HNE-protein adducts. Lane 1 is α-crystallin not treated with HNE. Lanes 2-5 are α-crystallins exposed to 25, 50, 100, and 200 μM HNE, respectively, for 2 h at 37°C. Lower panel) Protein profiles of α-crystallins isolated from bovine lenses after incubation with HNE (Coomassie Blue staining). Upper panel) modification of α-crystallins by HNE as determined by Western blotting with antibodies to HNE-modified proteins.