FIGURE 3.

Distribution of ubiquitin in the lens. The epithelium and differentiating fibers were stained with monoclonal antibodies against ubiquitin conjugates (A), showing primarily a nuclear staining. Double staining of the nuclei on fiber cells with polyclonal antibodies to ubiquitin (B) and monoclonal antibodies to lamin B (C) show that ubiquitin was present in nuclei in all stages of denucleation, even when the nuclear membrane was fragmented, as shown by the immunostaining of lamin B. For the negative control, primary antibodies were replaced with preimmune IgG (D). Scale bars, 50 μm.