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. Author manuscript; available in PMC: 2006 Feb 27.
Published in final edited form as: Plant J. 2005 Apr;42(1):84–94. doi: 10.1111/j.1365-313X.2005.02354.x

Figure 5. ATHB15 mRNA cleavage by miR166 in Nicotiana benthamiana, wheat germ extract, and in Arabidopsis.

Figure 5

(a) ATHB15 mRNA cleavage in N. benthamiana. Total RNAs were extracted from plants without any vector constructs (1), with pBI121 control alone (2), with pBI121 + miR166 vector (3), with pBI121 + ATHB15 vector (4), with pBI121 + ATHB15 vector + miR166 vector (5), with pBI121 + mATHB15 vector (6), and with pBI121 + mATHB15 vector + miR166 vector (7). RNA gel blots were probed with the 5′-end cleavage fragment of ATHB15. The arrow marks the full-size mRNA, and the arrowhead the 5′-end cleavage product.

(b) ATHB15 mRNA cleavage in wheat germ extract. A 5′-end labeled ATHB15- specific RNA was used in the assay.

(c) 5′-RACE to determine the cleavage site in Arabidopsis. The arrow in the diagram marks the cleavage site. The number refers to that of independent clones with the 5′-end as determined.