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. Author manuscript; available in PMC: 2006 Feb 27.
Published in final edited form as: J Biol Chem. 2005 Mar 23;280(22):21004–21014. doi: 10.1074/jbc.M413191200

Fig. 2.

Fig. 2

COL1A1 promoter activity is sensitive to methylation. Three COL1A1 promoter-reporter constructs (−112, −311, and −804) were treated with (M) or without (U) Sss1 methylase and transfected separately into IMR-90 cells as described under “Methods and Materials.” Luciferase activities were normalized by both protein concentration and green fluorescent protein fluorescence. Each experiment was repeated three times and values represented mean ± S.D. Statistical significance was assessed by one-sample two-tailed t test. Methylase treatment significantly inhibited the activities of all three COL1A1 promoter constructs (**, p < 0.01).