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. Author manuscript; available in PMC: 2006 Feb 27.
Published in final edited form as: J Biol Chem. 2005 Mar 23;280(22):21004–21014. doi: 10.1074/jbc.M413191200

Fig. 7.

Fig. 7

Inhibition of IFN-γ-induced expression of CIITA leads to partial alleviation of collagen repression. A, CIITA protein levels (top panel) and mRNA levels (lower graph) are decreased by CSH4 and CSH6 shRNAi. RNAi experiments were performed as described under “Materials and Methods.” Each experiment was repeated at least three times and a representative figure of CIITA Western blot and mRNA each was shown. B, top panel, collagen (COL1A1) mRNA levels are not repressed by IFN-γ in the presence of CSH4 and CSH6 shRNA. RNAi experiments were performed as described under “Materials and Methods.” Bottom panel, average COL1A1 mRNA level was plotted versus the CIITA protein level in four experiments performed in duplicate. Linear regression curve demonstrates significant correlation between high levels of CIITA and low levels of COL1A1.