Table III.

Primers for real-time PCR specific for human genes

Gene	Amplicon location (GenBank accession number)	Exon	Sequences
Human COL1A1 mRNA	201–1735 (NC_000017)
Forward primer		Exon1	5′-CAGGTCTCGGTCATGGTACCT-3′
Taqman probe		Exon1/2	6FAM-CATCCCACCAATCACCTGCGTACAGA-TAMRA
Reverse primer		Exon2	5′-GTCGAGGGCCAAGACGAA-3′
Human COL1A2 mRNA	7568–8965 (AF004877)
Forward primer		Exon5	5′-GCCCCCCAGGCAGAGA-3′
Taqman probe		Exon5/6	6FAM-CCTGGTCTCGGTGGGAACTTTGCTG-TAMRA
Reverse primer		Exon6	5′-CCAACTCCTTTTCCATCATACTGA-3′
Human type III CIITA mRNA	156–218 (AF410154)
Forward primer		Exon1	5′-ACGCCCTGCTGGGTCC-3′
Taqman probe		Exon1/2	6FAM-ACCTGTCAGAGCCCCAAGGCAGC-TAMRA
Reverse primer		Exon2	5′-AACTCCATGGTGGCACACTG-3′
Human HLA-DRA mRNA	341–427 (NM01911)
Forward primer		Exon2	5′-GCCAACCTGGAAATCATGACA-3′
Taqman probe		Exon2/3	6FAM-CAACTATACTCCGATCACCAATGTACCTCCAGAG-TAMRA
Reverse primer		Exon3	5′-AGGGCTGTTCGTGAGCACA-3′