Table 2.
Embryonic lethality and embryonic cell division defects after depletion of different actin isoforms by 3′UTR-specific RNAi
| Maternal genotype | % Embryonic lethality (15°C) | Early embryonic cell division defects |
|---|---|---|
| N2 | 0 (395) | No (n = 30) |
| act-2(ok1229) | 0 (310) | No (n = 12) |
| act-1(3′UTR RNAi) | 0 (175) | No (n = 10) |
| act-3(3′UTR RNAi) | 0 (185) | No (n = 12) |
| act-1(3′UTR RNAi); act-2(ok1229) | 100 (415) | Yes (n = 11) |
| act-3(3′UTR RNAi); act-2(ok1229) | 100 (345) | Yes (n = 13) |
| act-1(3′UTR RNAi); act-3(3′UTR RNAi) | 0 (196) | No (n = 8) |
| act-1(3′UTR RNAi); act-3(3′UTR RNAi);act-2(ok1229) | 100 (230) | Yes (n = 14) |
| act-4(3′UTR RNAi); act-5(3′UTR RNAi);act2(ok1229) | 0 (178) | No (n = 8) |
Individually plated L4 animals were shifted to the indicated temperature for 24 h at which time the adult was removed. Twenty-four to 36 h later the plates were scored for embryonic lethality.