Table 2.
Strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| AH109 | MATa, trp1-901, leu2-3 112, ura3-52, his3-200, gal4Δ, gal80Δ, LYS2::GAL1UAS-GAL1TATA-HIS3, GAL2UAS-GAL2TATA-ADE2, | Clontech |
| URA3:: MEL1UAS-MEL1 TATA-lacZ | ||
| BESY49-6C | MATacyh2r NDC10-YFP::kanMX6 | Yeast Resource Center |
| BESY46-1D | MATα cyh2r NDC10-CFP::hphMX3 | Yeast Resource Center |
| BSY9 | MATa/MATα ade2-1oc/ade2-1oc ADE3/ade3Δ can1-100/can1-100 CYH2s/cyh2r; his3-11,15/his3-11,15 leu2-3,112/leu2-3,112 trp1-1/trp1-1 ura3-1/ura3-1 | Hazun et al. (2003) |
| PWY8 | MATa/MATα ade3Δ/ade3Δ BIR1-Venus::kanMX6/BIR1-Venus::kanMX6 CYH2s/cyh2r NDC10-CFP:kanMX6/NDC10-CFP:kanMX6 | This study |
| PWY16-4D | MATaade3Δ bir1Δ::hphMX3 lys2Δ::HIS3 with autonomous plasmid pPW06 | This study |
| PWY19 | MATa/MATα ade3Δ/ade3Δ BIR1-Venus::kanMX6/BIR1-Venus::kanMX6 CYH2s/cyh2r SLI15-CFP::kanMX6/SLI15-CFP::kanMX6 | This study |
| PWY56 | MATaBIR1::bir1-876stop-Venus::kanMX6::URA3 | This study |
| PWY57 | MATaBIR1::bir1-ΔMN-Venus::kanMX6::URA3 | This study |
| PWY60 | MATaBIR1::BIR1-Venus::kanMX6::URA3 | This study |
| PWY61-4D | MATaade3Δ BIR1-TAP::kanMX6 cyh2r | This study |
| PWY75-6C | MATaade3Δ BIR1-Venus::kanMX6 NDC10-CFP::hphMX3 | This study |
| PWY76-1C | MATaade3Δ bir1-ΔIAP-Venus::kanMX6 NDC10-CFP::hphMX3 | This study |
| PWY77-2B | MATaade3Δ bir1-ΔBclB-Venus::kanMX6 NDC10-CFP::hphMX3 | This study |
| PWY78-6C | MATaade3Δ bir1-ΔSN-Venus::kanMX6 NDC10-CFP::hphMX3 | This study |
| PWY79-5A | MATaade3Δ bir1-ΔMB-Venus::kanMX6 NDC10-CFP::hphMX3 | This study |
| PWY80-2C | MATaade3Δ bir1-ΔMS-Venus::kanMX6 NDC10-CFP::hphMX3 | This study |
| PWY81-1B | MATaade3Δ bir1-ΔBS-Venus::kanMX6 lys2Δ::HIS3 NDC10-CFP::hphMX3 | This study |
| PWY82-2B | MATaade3Δ bir1-Venus::kanMX6 SLI15-CFP::hphMX3 | This study |
| PWY83-2A | MATaade3Δ bir1-ΔIAP-Venus::kanMX6 SLI15-CFP::hphMX3 | This study |
| PWY84-4D | MATaade3Δ bir1-ΔBclB-Venus::kanMX6 SLI15-CFP::hphMX3 | This study |
| PWY85-4D | MATaade3Δ bir1-ΔSN-Venus::kanMX6 SLI15-CFP::hphMX3 | This study |
| PWY86-1D | MATα ade3Δ bir1-ΔMB-Venus::kanMX6 SLI15-CFP::hphMX3 | This study |
| PWY87-5C | MATaade3Δ bir1-ΔMS-Venus::kanMX6 cyh2r SLI15-CFP::hphMX3 | This study |
| PWY88-10A | MATaade3Δ bir1ΔBS-Venus::kanMX6 cyh2r lys2Δ::HIS3 SLI15-CFP::hphMX3 | This study |
| PWY91-10C | MATaade3Δ bir1Δ::hphMX3 lys2Δ::HIS3 NDC10-YFP::kanMX6 with autonomous plasmid pPW06 | This study |
| PWY93-3B | MATaade3Δ BIR1—13xMyc::kanMX6 | This study |
| PWY160-1B | MATα ade3Δ NDC10-3xHA::kanMX6 | This study |
| PWY192 | MATaade3Δ bir1Δ::hphMX3::bir1-2xA-13xMyc::kanMX6::URA3 lys2Δ::HIS3 NDC10-YFP::kanMX6 | This study |
| PWY194 | MATaade3Δ bir1Δ::hphMX3:: bir1-9xA-13xMyc::kanMX6::URA3 lys2Δ::HIS3 NDC10-YFP::kanMX6 | This study |
| PWY199-5B | MATaade3Δ BIR1-13xMyc::kanMX6 cdc15-2 SLI15-3xHA::kanMX6 | This study |
| PWY200-14C | MATabir1-ΔIAP::hphMX3 his3-11::PCUP1-GFP12-lacI12::HIS3 mcd1-1 PDS1-3xHA:URA3 trp1-1::lacO::TRP1 | This study |
| PWY200-17A | MATahis3-11::PCUP1-GFP12-lacI12::HIS3 mcd1-1 PDS1-3xHA::URA3 trp1-1::lacO::TRP1 | This study |
| PWY202-11B | MATaBIR1-13xMyc::kanMX6 cdc15-2 NDC10-3xHA::kanMX6 | This study |
| PWY204 | MATaade3Δ bir1Δ::hphMX3::bir1-ΔIAP-13xMyc::kanMX6:URA3 lys2Δ:HIS3 | This study |
| PWY205 | MATaade3Δ bir1Δ:hphMX3:bir1-ΔBclB-13xMyc:kanMX6:URA3 lys2Δ:HIS3 | This study |
| PWY206 | MATaade3Δ bir1Δ:hphMX3:bir1-ΔSN-13xMyc:kanMX6:URA3 lys2Δ:HIS3 | This study |
| PWY207 | MATaade3Δ bir1Δ:hphMX3:bir1-ΔMB-13xMyc:kanMX6:URA3 lys2Δ:HIS3 | This study |
| PWY208 | MATaade3Δ bir1Δ:hphMX3:bir1-ΔMS-13xMyc:kanMX6:URA3 lys2Δ:HIS3 | This study |
| PWY209 | MATaade3Δ bir1Δ:hphMX3:bir1-ΔBS-13xMyc:kanMX6:URA3 lys2Δ:HIS3 | This study |
| JLY15 | MATa/MATα ADE3/ade3Δ BIR1-Venus:kanMX6/BIR1-Venus:kanMX6 cyh2r/cyh2r; his3-11/HIS3 HIS4/his4:CYH2s | This study |
| JLY16 | MATa/MATα ADE3/ade3Δ bir1-ΔIAP-Venus:kanMX6/bir1-ΔIAP-Venus:kanMX6 cyh2r/cyh2r; his3-11/HIS3 HIS4/his4:CYH2s | This study |
| JLY17 | MATa/MATα ADE3/ade3Δ bir1-ΔBclB-Venus:kanMX6/bir1-ΔBclB-Venus:kanMX6 cyh2r/cyh2r HIS3/HIS3 HIS4/his4:CYH2s lys2Δ:HIS3/LYS2 | This study |
| JLY18 | MATa/MATα; ADE3/ADE3 bir1-ΔSN-Venus:kanMX6/bir1-ΔSN-Venus:kanMX6 cyh2r/cyh2r HIS3/his3-11,15 his4:CYH2s/HIS4 | This study |
| JLY19 | MATa/MATα ADE3/ADE3 bir1-ΔMB-Venus:kanMX6/bir1-ΔMB-Venus:kanMX6 cyh2r/cyh2r his3-11,15/HIS3 HIS4/his4:CYH2s | This study |
| JLY20 | MATa/MATα ade3Δ/ADE3 bir1-ΔMS-Venus:kanMX6/bir1-ΔMS-Venus:kanMX6 cyh2r/cyh2r HIS3/his3-11,15 his4:CYH2s/HIS4 | This study |
| JLY21 | MATa/MATα ade3Δ/ADE3 bir1-ΔBS-Venus:kanMX6/bir1-ΔBS-Venus:kanMX6 cyh2r/cyh2r HIS3/HIS3 his4:CYH2s/HIS4 LYS2/lys2Δ:HIS3 | This study |
| TDY93 | MATa/MATα ADE3/ade3Δ cyh2r/cyh2r his3-11,15/HIS3 HIS4/his4:CYH2r | Davis (1992) |
| W303 | MATaade2—1oc can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 |
All strains, except strain AH109, have the same markers as W303 except as shown.