Figure 4.

Nmnat1 is not sufficient to target Wld^S protein to intranuclear foci. C-terminal EGFP fusion constructs transiently transfected into COS cells. (A) Wld^S/EGFP localizes primarily to discrete intranuclear foci (arrowhead), although some remains homogeneously distributed inside the nucleus (arrow). (B) Nmnat1/EGFP is homogeneously distributed, apart from exclusion from nucleoli. N70/EGFP (C) and EGFP (D) alone are homogeneously distributed throughout the cell but EGFP has a preference for the nucleus as previously described (Alonso et al., 2004). Neither is targeted to intranuclear foci. Scale bar, 10 μm. Figures are representative of three experiments.