Figure 8.

Syk KD is able to reduce Stx endocytosis and trafficking to the Golgi apparatus. HeLa cells were transfected 24 h before the experiment. They were then washed and incubated with Stx (250 ng/ml) for 45 min at 37°C. After fixation, the cells were visualized by confocal microscopy. Transfected cells were distinguished by using anti-Syk antibodies (red channel), and Stx was revealed by using anti-Stx antibodies (green channel). The transfected cells take up less Stx and also have less toxin in the Golgi area. In the panels to the right, Stx is shown in gray because this makes it easier to see the effect of the expression of Syk KD on its transport.