Table 2.
Associations between DDX1 bodies, cleavage bodies, Cajal bodies, and gems in HeLa PV cells during the cell cycle
| % DDX1 bodiesd,e
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Cell cycle phasea | DDX1 bodies (DDX1)b | Cleavage bodies (CPSF-100)b | CBs (Sm)b | Gems (SMN)b | % DDX1 bodies associated with cleavage bodiesc,d | Associated with CBs | Associated with gems | % CBs associated with gemsd,f | % DDX1 bodies associated with CBs and gemsd,g |
| Early G1 (11 h) | 0.2 (0-2) | ND | 1.5 (0-3) | 2.6 (0-7) | ND (0/14) | ND (0/8) | ND (0/8) | 35 (19/54) | ND (0/14) |
| Mid-S (3 h) | 0.6 (0-2) | 0.2 (0-2) | 1.5 (0-3) | 2.0 (0-4) | 24% (5/21) | 42% (11/26) | 38% (10/26) | 41 (21/51) | 31 (8/26) |
| Early G2 (6 h) | 0.5 (0-2) | 0.2 (0-1) | 1.7 (0-4) | 2.7 (0-5) | 21% (3/14) | 67% (12/18) | 61% (11/18) | 67 (39/58) | 61 (11/18) |
A minimum of 35 cells were three-dimensionally reconstructed and examined for each triple-labeling experiment at each time point. ND, not detected.
Time after release in parentheses.
Average number of nuclear bodies per cell with the range of nuclear bodies in cells analyzed in parentheses.
Percentage of DDX1 bodies that are associated with cleavage bodies (shown by CPSF-100 staining), with colocalization being the predominant pattern (∼60% of associations).
Parentheses indicate number of associations per total number of nuclear bodies examined.
Percentage of DDX1 bodies that are associated with CBs or gems, with adjacent localization being the predominant pattern (∼70% of associations).
Percentage of CBs that are associated with gems, with adjacent localization being the predominant pattern (∼70% of associations).
Percentage of DDX1 bodies that are associated with CBs and gems, with adjacent localization being the predominant pattern (∼70% of associations).