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. 2005 Dec 19;171(6):967–979. doi: 10.1083/jcb.200504104

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Copyright © 2005, The Rockefeller University Press

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Figure 3. — Nuclear import of Pik1 requires importin-β, but not importin-α. (A) An importin-α (kap60 ^ts) mutant (YTS0012 srp1-31 ^ts) and its otherwise isogenic KAP60 ⁺ parent (W303-1a) carrying pTS7 were grown in SCRaf-Trp at 26°C to A_{600 nm} ≈ 0.7, and a portion of each culture was shifted to 37°C. After 1 h, expression of GFP-Pik1 was induced by addition of galactose, and the cells were incubated for an additional 2 h at the indicated temperature before being inspected by fluorescence microscopy. (B) The identical experiment described in A was performed with an importin-β (kap95 ^ts) mutant (SWY1313 rsl1 ^ts) and its otherwise isogenic parent strain (SWY1312) carrying pTS7, except that, after galactose induction, the cells were incubated for 3 h at the indicated temperature before inspection.