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. 1996 Mar;38(3):426–429. doi: 10.1136/gut.38.3.426

K-ras gene mutation in gall bladder carcinomas and dysplasia.

T Ajiki 1, T Fujimori 1, H Onoyama 1, M Yamamoto 1, S Kitazawa 1, S Maeda 1, Y Saitoh 1
PMCID: PMC1383074  PMID: 8675098

Abstract

Epithelial dysplasia of gall bladder is an important precancerous lesion of gall bladder carcinogenesis. To investigate the frequency of K-ras gene mutation in gall bladder carcinoma and dysplasia, K-ras codon 12 mutations were investigated by the polymerase chain reaction/restriction enzyme based method following direct sequencing. Mutation was detected in 59% (30 of 51) of gall bladder carcinomas, in 73% (8 of 11) of gall bladder dysplasia in gall stone cases, and in 0% of the normal gall bladder epithelium. There was, however, no correlation between K-ras mutation and clinicopathological factors of gall bladder carcinoma. K-ras gene mutation occurs even in gall bladder dysplasia at an incidence similar to that in carcinomas, suggesting that testing for K-ras gene mutation may prove useful as an adjunct to bile cytological or biopsy analysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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