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. Author manuscript; available in PMC: 2006 Aug 1.
Published in final edited form as: J Histochem Cytochem. 2005 May 6;53(8):1011–1021. doi: 10.1369/jhc.4A6599.2005

Figure 6.

Figure 6

Upregulation of the eCLCA1 mRNA (A) 35S-exposure of a Northern blot) and protein (B) Western blot probed with antibody α-eCa1 or preimmune serum (PI) in equine lung tissue with recurrent airway obstruction (RAO). (A) The eCLCA1 mRNA was undetectable in the normal equine lung whereas a strong signal was obtained from a lung with RAO. (B) Likewise, the processed eCLCA1 protein of ~80 kDa was only weakly detectable in the lung of a healthy horse, whereas a strong 80-kDa signal and a weaker 120-kDa signal were detected in the lung of a horse with RAO. EF-1a, housekeeping mRNA elongation factor-1a.