Table 1.
Overview of strains tested for salt perception
| Gene | Reference | Function | Taste | Plasticity |
|---|---|---|---|---|
| arr-1 (ok401) | Fukuto et al (2004) | Arrestin | WT | −0.12±0.17*** |
| ceh-36 (ks86) | Lanjuin et al (2003) | Transcription factor | m*** | 0.13±0.03*** |
| che-1 (p679) | Uchida et al (2003) | Transcription factor | m | 0.01±0.02*** |
| cnb-1 (jh103) | Bandyopadhyay et al (2002) | Calcineurin B | m* | 0.74±0.04** |
| daf-11 (m47) | Birnby et al (2000) | Gyanylate cyclase | WT | WT |
| dgk-1 (sy428) | Hadju-Cronin et al (1999); Nurrish et al (1999) | Diacylglycerol kinase | WT | 0.72±0.05*** |
| (nu62) | WT | 0.27±0.10*** | ||
| egl-4 (ky95) | Daniels et al (2000) | PKG | WT | 0.06±0.06*** |
| (ok1105) | WT | −0.06±0.08*** | ||
| fat-1 (wa9) | Watts and Browse (2002) | ω-3 fatty acyl desaturase | WT | −0.02±0.16** |
| fat-3 (wa22) | Watts and Browse (2002) | δ-6 fatty acid desaturase | WT | 0.20±0.06*** |
| fat-4 (wa14) | Watts and Browse (2002) | δ-5 fatty acid desaturase | WT | 0.31±0.09*** |
| (ok958) | WT | 0.10±0.03*** | ||
| gcy-32 (ok995) | Coates and de Bono (2002) | Guanylate cyclase | WT | WT |
| gcy-35 (ok769) | Cheung et al (2004) | Gyanylate cyclase | WT | −0.10±0.10* |
| gpa-1 (pk15) | Jansen et al (1999) | Gα subunit | WT | 0.17±0.06** |
| gpa-2 (pk16) | Zwaal et al (1997) | Gα subunit | WT | WT |
| gpa-3 (pk35) | Zwaal et al (1997) | Gα subunit | WT | WT |
| gpa-4 (pk381) | Jansen et al (1999) | Gα subunit | WT | WT |
| gpa-10 (pk362) | Jansen et al (1999) | Gα subunit | WT | WT |
| gpa-11 (pk349) | Jansen et al (1999) | Gα subunit | WT | WT |
| gpa-13 (pk1270) | Jansen et al (1999) | Gα subunit | WT | WT |
| gpa-14 (pk347) | Jansen et al (1999) | Gα subunit | WT | WT |
| gpa-15 (pk477) | Jansen et al (1999) | Gα subunit | WT | WT |
| grk-2 (rt97) | Fukuto et al (2004) | GRK | m*** | −0.61±0.06***,# |
| itr-1 (sa73) | Baylis et al (1999); Dal Santo et al (1999) | IP3 receptor | WT | 0.82±0.06*** |
| ncs-1 (qa406) | Gomez et al (2001) | Ca2+ sensor | m** | −0.13±0.14** |
| ocr-1 (ok132) | Tobin et al (2002) | TRPV channel | WT | −0.20±0.08*** |
| (ak46) | WT | −0.25±0.14*** | ||
| ocr-2 (ak47) | Tobin et al (2002) | TRPV channel | WT | −0.18±0.12*** |
| odr-3 (n1605) | Roayaie et al (1998) | Gα subunit | WT | 0.85±0.04*** |
| osm-9 (ky10) | Colbert et al (1997) | TRPV channel | WT | 0.06±0.05** |
| pdl-1 (gk157) | Smith and Pilgrim, personal communication | Phosphodiesterase | WT | 0.13±0.09** |
| tax-2 (p671) | Coburn and Bargmann (1996) | cGMP-gated channel | m*** | 0.83±0.04*** |
| tax-4 (p678) | Komatsu et al (1996) | cGMP-gated channel | m*** | 0.83±0.03*** |
| tax-6 (p675) | Kuhara et al (2002) | Calcineurin A | m*** | 0.64±0.05*** |
| In all, 32 mutant strains were tested for chemotaxis to 0.1, 1, 10 and 100 mM NaCl (taste) or chemotaxis to 25 mM NaCl after pre-exposure to 100 mM NaCl (plasticity) and their responses were compared to wild-type animals: *P<0.05; **P<0.01; ***P<0.001; unc=locomotion defect; these strains did not perform well in the assay; #pre-exposure for only 5 min, response stronger than wild types. WT=wild type. | ||||