Table 1.
IgVλ mutations in [hSMUG1] and control transfectants in DT40X2U
| Cells |
IgVλ sequencesa |
Mutation typeb |
Mutation load | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Expansion (week) | % sIgM− | Total (n) | Mutated (%) | Total (n) | Independent (n) | Transversions at C:G (n) | % Tvnsc | Mutations/mutated sequence | |
| hSMUG1 transfectant | |||||||||
| c1 | 8 | 0.5 | 87 | 26 | 32 | 19 | 18 | 72 | 1.4 |
| c2 | 8 | 2.5 | 43 | 51 | 32 | 23 | 22 | 46 | 1.5 |
| c3 | 10 | 6 | 57 | 28 | 20 | 15 | 15 | 53 | 1.3 |
| c4 | 10 | 2.8 | 67 | 78d | 73 | 20 | 18 | 50 | 1.4 |
| Pooled | 46 | 77 | 73 | 55 | 1.4 | ||||
| Vector-only transfectant | |||||||||
| c1 | 8 | 9.1 | 48 | 88 | 102 | 57 | 54 | 13 | 2.4 |
| c2 | 8 | 9 | 47 | 87 | 97 | 56 | 56 | 9 | 2.4 |
| c3 | 8 | 6 | 62 | 92 | 179 | 76 | 67 | 12 | 3.1 |
| Pooled | 89 | 189 | 177 | 11 | 2.6 | ||||
| aSequences obtained from subpopulations (2%) sorted as giving the dullest staining with anti-IgM. | |||||||||
| bAll the independent 266 mutational events observed in this study were single nucleotide substitutions except for six possible gene conversions and four deletions. | |||||||||
| cThe percentage of substitutions at C:G base pairs that are nucleotide transversions. | |||||||||
| dOne jackpot mutation in this clone was present in most of the sequences obtained. | |||||||||