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PACT binds to hAgo2. (A) Immunoprecipitation followed by Western blotting. FLAG-PACT or FLAG-TRBP proteins, V5-Dicer and V5-hAgo2 protein were coexpressed in HEK293T cells. Immunoprecipitation was carried out using anti-FLAG antibody and the protein was visualized using either anti-V5 or anti-FLAG antibody. (B) The same experiment as described in (A), except for using FLAG-PACT mutants and V5-hAgo2 (wild type). In this experiment, V5-Dicer was not coexpressed.
