Table 2.
Mutants identified from genomic deletion screen with Deg1-Ura3-HA3
| Systematic name | Standard name | RGa | Functional description |
|---|---|---|---|
| Ubiquitin conjugation | |||
| YMR022W | UBC7 | 4 | Ubiquitin-conjugating enzyme |
| YMR264W | CUE1 | 4 | Recruits Ubc7 to the ER |
| YIL030C | DOA10 | 4 | E3 ubiquitin ligase |
| Proteasome | |||
| YDL020C | RPN4 | 2 | Transcriptional activator for proteasome genes |
| YBR173C | UMP1 | 3 | 20S proteasome maturation factor |
| YDR363W-A | SEM1 | 3 | 26S proteasome regulatory subunit |
| Chromosome/cell cycle/DNA replication | |||
| YGR188C | BUB1 | 3 | Protein kinase that functions in spindle checkpoint |
| YOR026W | BUB3 | 3 | Spindle checkpoint protein; binds Bub1 |
| YPL008W | CHL1 | 2 | Helicase, required for sister chromatid cohesion |
| YPR141C | KAR3 | 3 | Kinesin related, required for sister chromatid cohesion |
| YHR191C | CTF8 | 2 | Replication factor, required for sister chromatid cohesion |
| Telomerase | |||
| YLR233C | EST1 | 2 | Telomerase holoenzyme subunit |
| YLR318W | EST2 | 2 | Telomerase catalytic subunit |
| YIL009C-A | EST3 | 2 | Telomerase holoenzyme subunit |
| Other | |||
| YPR131C | NAT3 | 3 | Catalyzes acetylation of N-terminal methionine of proteins |
| YJL092W | SRS2 | 3 | DNA helicase, disassembles Rad51 filaments |
| aRelative growth value was based on three to four independent growth assays (scales 1–4, 4=fastest). | |||