Table 1.

Antigen and integrin ligand affinities

Antigen	KA (× 106 M−1)	Integrin ligand	KA (106 M−1)
3-83 binding		VCAM-1
p31	65	High-affinity VLA-4	10
p11	7	Low-affinity VLA-4	∼0.1
p0	—
HyHEL10 binding		ICAM-1
HELWT	50 000	High-affinity LFA-1	∼2
HELRD	2080	Low-affinity LFA-1	∼0.01
HELKD	30
HELRKD	∼3
The KA values for the different peptides/3-83 antibody and HEL mutants/HyHEL10 antibody were obtained using the Biacore, as previously described (Batista and Neuberger, 1998; Carrasco et al, 2004). The values derived represent the affinity of the dimeric antibody for the peptide in the case of 3-83. The values of HyHEL10 for HELWT and the different HEL mutants account for the monomeric interaction. The KA values for the integrins VLA-4 (Chigaev et al, 2003) and LFA-1 (Lollo et al, 1993) in high- and low-affinity states were previously reported.