Figure 7.

Effects of expression of LIMK1 mutants on VEGF-induced cell migration and stress fiber formation. (A) Effects on cell migration. Migration of MSS31 cells expressing YFP alone (control) or YFP plus Myc-LIMK1 mutant was analyzed by Transwell assays. Relative numbers of migrating cells are shown as means±s.d. of four experiments. (B) Expression levels of LIMK1 mutants. MSS31 cells were transfected as above, and cell lysates were immunoprecipitated and immunoblotted with anti-LIMK1 antibody. (C) Effects on stress fiber formation. MSS31 cells transfected with Myc-LIMK1 mutants were stimulated with or without VEGF for 15 min. The cells were costained with rhodamine-phalloidin and anti-Myc antibody. Arrowheads indicate the cells expressing Myc-LIMK1 mutant (see Supplementary Figure 3D). Bar, 50 μm. The right panel shows the data of quantitative analysis of triplicate experiments.