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. 2006 Feb 9;25(4):774–784. doi: 10.1038/sj.emboj.7600978

Figure 4.

Figure 4

FRET analysis between PLCγ1-YFP and various CFP-conjugated proteins. (A) Jurkat E6 cells expressing PLCs-YFP and LAT-CFP were seeded on a stimulatory coverslip and fixed 2 min into the spreading process. FRET efficiency values are presented in a pseudo-colored scale, with black color representing saturated pixels. The average value of FRET efficiency±s.e. obtained for a given pair is shown. The size bar corresponds to 5 μm. (B) Same as (A) for cells expressing PLCs-YFP and c-Cbl-CFP. (C) Same as (A) for cells expressing PLCs-YFP and Vav1-CFP. (D) Same as (A) for cells expressing PLCs-YFP and CFP-Slp76. (E) Same as (A) for cells expressing PLCs-YFP and Slp76-CFP. (F) Same as (A) for cells expressing PLCs-SH2N*-YFP and LAT-CFP.