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Measurement of RNA–RNA binding. The increase in anisotropy of 5′ fluorescently labeled P5.1 RNA was measured as a function of the concentration of cognate RNAs. (A) Binding of P5.1 to Δ5.1 mutant RNase P RNA. (B) Binding of P5.1 RNA to its isolated cognate stem–loop, P15.1. (C) Binding of P5.1 RNA to aptamer 7C.
