FIGURE 6.

U7-Slbp double mutant analysis. RNA isolated from the indicated genotypes was subjected to Northern analysis with a [³²P]-labeled H3 probe (A,C) or S1 nuclease protection analysis with a [³²P]-labeled H2a probe (B). (A) H3 Northern of third instar larvae. (Lanes 1– 5) RNA from the indicated homozygous single or double mutant genotypes. (Lane 6) w¹¹¹⁸ control RNA. (B) H2a S1 nuclease protection analysis of third instar larvae. (Lane 1) [³²P]-labeled marker (M) RNAs of the indicated lengths. (Lanes 2–6) RNA from the indicated homozygous single or double mutant genotypes. (Lane 7) w¹¹¹⁸ control RNA. (C) (Lane 1) RNA from embryos collected overnight from heterozygous parents. Note that one-quarter of the embryos will be homozygous mutant for the indicated genotypes. (Lanes 2–4) RNA from first, second, and third instar larvae, respectively, homozygous mutant for the indicated genotypes. (Lane 5) Control RNA from w¹¹¹⁸ third instar larvae.