FIGURE 1.

Primer extension analysis of RNA extracted from putative RNA methyltransferase deletion strains. Total RNA was annealed to a [³²P]-labeled primer complementary to residues 1506–1525 in 16S rRNA, prior to extension by M-MLV-RT, as described in Materials and Methods. Products of the reaction were separated on 8% acryl-amide/7 M urea denaturing gels and visualized by autoradiography. A stop at position A1499, noted by an arrow, indicates the presence of a methyl group at U1498.