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Dehydration-induced HPR1 circularization without freezing. Solutions of linear, internally 32P-labeled HPR1 in 25 mM Na-acetate and 10 mM Na3EDTA at pH 8.0 were incubated for 1 h at 25°C under the conditions indicated and analyzed as in Figure 2 ▶. (A) Effect of ethanol. (B) (Lanes 1–6) Effect of evaporation under vacuum of solution samples containing the indicated concentrations of poly(ethylene glycol). (Lanes 7–12) Same as 1–6 but without evaporation. MM, putative multimers.
