TABLE 2.

EMSA binding of PTB1 constructs to GABA RNAs

	I/E	I	I34	I11	I11/E	Exon	I11/Em
PTB1	2 nM (4)	1 nM (3)	1 nM (3)	3 nM (1)	15 nM (3)	0	3 nM (3)
PTB1:12	7 nM (3)	70 nM (3)	16 nM (2–3)	0	~500 nM (1)	0	190 nM (2)
PTB1:23	90 nM (3)	450 nM (1–2)	nd	0	90 nM (2)	0	nd
PTB1:34	1 nM (4)	5 nM (2)*	8 nM (3)	84 nM (1)	4 nM (3–4)	0	4 nM (>3)*

Protein concentrations are given that correspond to the first appearance of a shifted band on the gel; they are not dissociation constants. (nd) Not determined; (0) no binding observed at protein concentrations from nanomolar to 3μM. Numbers in parentheses are the number of discrete shifted bands observed. (*) Bands very diffuse, with no clear boundaries.