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. 2006 Mar;12(3):476–487. doi: 10.1261/rna.2243206

FIGURE 6.

FIGURE 6.

RNAi analysis suggesting the UTP effect involves TbMP57. (A–C) RNAi to TbMP57. (A) Northern blot showing TbMP57 mRNA (mRNA) and TbMP57 double-stranded RNA (dsRNA) from TbMP57 RNAi (p57i) and control (Co; 29.13) cells at 24 h post-induction. (B) Western blot showing TbMP57 protein (p57) and a loading control protein (load) in various amounts of rapid cell lysate prepared from TbMP57 RNAi (p57i) and control (Co; 29.13) cells at 48 h post-induction. The numbers superimposed on the gel, under each lane, indicate the band intensity, relative to that of the control cells in the first lane. Below the gel is shown the fraction of TbMP57 protein remaining (remains) relative to the loading control protein for each lane. (C) UTP effect on the full round U-deletion reaction. Reactions were as in Figure 2A ▶, except using the rapid cell lysates of B. Below the final lane is shown the fraction of the UTP effect remaining in the TbMP57 RNAi (p57i) extract (remains) relative to that of the control (Co) extract. (D) UTP effect on full round U-deletion using TbTUT108 RNAi cells. Reactions were as in C, except using mitochondrial extract from cells in which TbTUT108 was depleted by RNAi (p108i).