FIGURE 9.

Comparison of TUTase efficiency. (A) TUTase activity in U-insertion. PPi-containing reactions using intact U-insertional substrate were as in Figure 3A ▶, except using gRNA I47G and no AMP-CP (see Materials and Methods). The positions of the 5′-end-labeled mRNA after cleavage at the U-insertion site (ins cut; 32 nt) and when extended by one or two U nucleotides (+1, +2) are indicated. (B) TUTase activity in U-deletion. PPi-containing reactions using intact U-deletional substrate, reproduced from Figure 3A ▶.