TABLE I.
Strains and plasmids used in this work
| Strain or plasmid | Genotype or relevant characteristics | Source or Ref. |
|---|---|---|
| E. coli | ||
| DH5α | F− φ80dlacZΔM15 ΔlacZYA-argF)U169 deoR recA1 endA1 hsdR17(rk− mk+) phoA supE44 λ−thi-1 gyrA96 relA1 | (57) |
| S. cerevisiae | ||
| KS106 | MATα ade2-1 can1-100 his3-11,15 leu2-3 112, trp1-1 ura3-1 cki1Δ::HIS3 eki1Δ::TRP1 | (61) |
| Plasmids | ||
| YEp351 | Multicopy E. coli/yeast shuttle vector containing LEU2 | (91) |
| pYY264 | CKI1 derivative of YEp351 | (49) |
| pYY265 | CKI1S30A derivative of pYY264 | (49) |
| pYY266 | CKI1S85A derivative of pYY264 | (49) |
| pCK25A | CKI1S25A derivative of pYY264 | This study |
| pCK25D | CKI1S25D derivative of pYY264 | This study |
| pCK37A | CKI1S37A derivative of pYY264 | This study |
| pCK25A/37A | CKI1S25A,S37A derivative of pCK25A | This study |
| pCK25A/30A | CKI1S25A,S30A derivative of pCK25A | This study |