Figure 1.

SDS–PAGE of the processed (truncated) recombinant RbbA protein. Lanes: M, Molecular weight markers; 1, Total E.coli lysate prior to IPTG induction; 2, E.coli lysates after IPTG induction; 3, An aliquot of supernatant after incubation with Ni-NTA resins; 4, An aliquot from the flow through of the NTA column used to purify the RbbA; 5, Eluted with 250 mM imidazole from the NTA column and finally dialyzed and concentrated RbbA protein.