Figure 2.

USF does not regulate transcription through the E-box. (A) HeLa cells were transfected with wild-type pFMR1-luc or with pFMR1-luc with point mutations in the E-box/CRE and luciferase activities were measured in cell lysates. (B) Either the wild-type (left) or E-box/CRE mutant reporter (right) was transfected into HeLa cells with or without pSV-USF1 and/or pSV-USF2 expression vectors as indicated. (C) pFMR1-luc or pHOGL3/4.5 was transfected into HeLa cells with the indicated amount of A-USF expression plasmid, or empty vector. Values for the FMR1 or HO-1 promoters were normalized to a CMV transfection control reporter. Data are expressed as percent normalized luciferase activity. Error bars represent ±1 standard deviation.