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P-CREB/ATF and CBP/p300 occupy human FMR1 in vivo. (A) ChIP was carried out with either anti-P-CREB antibody or (B) antibodies to CBP and p300, or non-specific rabbit IgG [N.S. in both (A and B)]. PCR was performed to amplify the FMR1 and c-fos promoters in normal and fragile X cells (left). Occupancy of p300 was quantified by real-time PCR of an independent set of triplicate IPs (right); error bars represent ±1 standard deviation.
