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. 2006 Feb 25;34(4):1205–1215. doi: 10.1093/nar/gkj521

Figure 7.

Figure 7

Stimulation of the human FMR1 promoter in Drosophila SL2 cells. (A) pFMR1-luc reporter plasmid was co-transfected into SL2 cells with an empty vector or vectors expressing NRF-1 (pACTIN-FL-NRF-1), or NRF-2 (both A5CΔP-hGABPα and A5CΔP-hGABPβ) or both NRF-1 and NRF-2. Firefly luciferase values are normalized to a Renilla luciferase control. The fold-changes are shown above each bar in relation to the sample representing pFMR1-luc alone which was set to 1×. Each bar represents the average of three transfections and error bars represent ±1 standard deviation, except for NRF-2 for which the average of two transfections was plotted. (B) pFMR1-luc, or pFMR1-luc with the NRF-2 or E-box/CRE sites mutated, were co-transfected with vectors expressing human ATF-1 (A5CΔP-hATF1), or NRF-2 (both A5CΔP-hGABPα and A5CΔP-hGABPβ) or both ATF-1 and NRF-2. The fold-changes are shown above each bar, in relation to their appropriate controls and are plotted in relative light units (RLU) on the vertical axis. Bars represent the averages of at least three transfections. Error bars for all samples represent ±1 standard deviation.